The antinuclear antibody (ANA) test is a standard screening assay for detecting multiple antibodies that may be produced by a patient with an autoimmune or ANA associated rheumatic disease (AARD). In addition, workflow benefits were observed which need to be analyzed in future studies. Excellent agreements were obtained between reading archived images on NOVA View and manually on a fluorescent microscope. Agreement of identifiable patterns between methods was 97%, with PCNA and mixed patterns undetermined. At a 1 : 40/1 : 80 dilution the resulting comparison demonstrated 94.8%/92.9% positive, 97.4%/97.4% negative, and 96.5%/96.2% total agreements between manual IIF and NOVA View archived images. Samples were read using the archived images on NOVA View and compared to results obtained from manual reading. In addition, 12 defined sera from the Centers for Disease Control and 100 random patient sera sent to ARUP Laboratories for ANA HEp-2 IIF testing were included. 369 well defined sera from: 44 rheumatoid arthritis, 50 systemic lupus erythematosus, 35 scleroderma, 19 Sjögren’s syndrome, and 10 polymyositis patients as well as 99 healthy controls were examined. To evaluate NOVA View with focus on reading archived images versus microscope based manual interpretation of ANA HEp-2 slides by an experienced, certified medical technologist.
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